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Polyoma Virus Middle T Gene Can Trigger Malignant Transformation of Early Passage Rodent Cells

^1* The Beatson Institute for Cancer Research, Garscube Estate, Bearsden, Glasgow G61 1BD, U.K.
and² Hellenic Institute Pasteur, 127 Vas Sofias Avenue, Athens, Greece

Previous studies on the tumourigenic conversion of early passage rat embryo cells by the polyoma virus early genes have suggested a multigenic control of tumourigenesis. Thus, the large T gene can immortalize early passage rat cells and can relieve the serum dependence of normal and transformed cells. The middle T gene alone cannot immortalize early passage cells; however, it can induce cells of established cell lines to become anchorage-independent and tumourigenic. Here we show that when linked to transcriptional enhancers, the polyoma virus middle T gene can trigger the complete malignant transformation of early passage rodent cells. Therefore, the polyoma virus middle T gene does not require a cooperating oncogene to induce malignant conversion of these cells.

Keywords: polyoma virus, malignant transformation, transfection, middle T

Received 18 November 1985; accepted 23 January 1986.