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J Gen Virol 67 (1986), 801-808; DOI 10.1099/0022-1317-67-4-801
© 1986 Society for General Microbiology
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Mode of Sensitivity and Resistance of Vaccinia Virus Replication to Interferon

Mariano Esteban, Javier Benavente and Eduardo Paez

Departments of Biochemistry, Microbiology and Immunology, Downstate Medical Center, Brooklyn, New York 11203, U.S.A.

In this study we show that vaccinia virus replication can be sensitive or resistant to interferon (IFN) in the same strain of mouse L cells. When IFN-treated L cells were maintained in suspension culture, infection led to a rapid inhibition of both viral and cellular protein synthesis together with breakdown of viral RNA and of rRNA. When IFN-treated L cells were maintained in monolayer culture, infection did not lead to significant inhibition of viral protein or RNA synthesis and breakdown of viral or of rRNA was not observed. The resistance of vaccinia virus replication to IFN was not dependent on the input multiplicity or state of growth of the cells (actively dividing or resting). Qualitative and quantitative differences in viral transcription and translation were observed between the two virus-cell systems. Our findings are consistent with the hypothesis that the sensitivity or resistance of vaccinia virus to IFN is mediated by specific viral products that act as activators or selective inhibitors of, at least, the dsRNA-dependent ppp(A2'p)nA synthetase/RNase system.

Keywords: vaccinia virus, gene expression, IFN

Received 31 July 1985; accepted 13 January 1986.




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Copyright © 1986 by the Society for General Microbiology.