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J Gen Virol 67 (1986), 851-862; DOI 10.1099/0022-1317-67-5-851
© 1986 Society for General Microbiology
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In vitro Transcriptase Deficiency of Temperature-dependent Host Range Mutants of Chandipura Virus

Nassar Rasool{dagger} and C. R. Pringle{ddagger}

Institute of Virology, Church Street, Glasgow G11 5JR, U.K.

Ten of 12 Chandipura virus tdCE mutants, which exhibit temperature-dependent restriction of growth in chick embryo (CE) cells but not in BS-C-1 cells, showed deficient transcriptase activity in vitro at 39 °C relative to wild-type virus. A gradation in transcriptional activity at 39 °C in vitro was observed. Reversion of the tdCE phenotype to unrestricted growth in CE cells at 39 °C was accompanied by partial restoration of normal transcriptase activity at 39 °C, suggesting that reversion was mediated by either extragenic or intragenic suppression. Viral protein synthesis was reduced or absent in CE cells at 39 °C indicating that transcription was also defective in vivo under these conditions. Induction of heat-shock proteins in CE cells at 39 °C occurred normally in tdCE mutant-infected cells and RNA methylation in vitro was unaffected.

Keywords: Chandipura virus, rhabdovirus, host range mutants, transcription

{dagger} Present address: Department of Genetics & Cellular Biology, University of Malaya, Kuala Lumpur 22-11, Malaysia.

{ddagger} Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

Received 21 October 1985; accepted 17 January 1986.




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