HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Crawford, A. M. Articles by Alfiler, A. R. Search for Related Content PubMed Articles by Crawford, A. M. Articles by Alfiler, A. R. Agricola Articles by Crawford, A. M. Articles by Alfiler, A. R.

Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

¹ Entomology Division, Department of Scientific and Industrial Research, Private Bag, Auckland, New Zealand
² UNDP/FAO Coconut Pests and Diseases Project, P.O. Box 1864, Manila, Philippines
and³ Philippine Coconut Authority, Albay Research Center, Guinobatan, Albay, Philippines

Twelve geographical isolates of Oryctes baculovirus were cloned in DSIR-HA-1179 cells. The DNA of these isolates was analysed by restriction endonuclease digestion with the enzymes BamHI, EcoRI, HindIII and PstI. Each isolate showed slightly different restriction fragment electrophoresis profiles. Most of the changes were due to small insertions or deletions of DNA, although two isolates lacked a single restriction site. The sites of genotypic change were not randomly distributed but were mainly associated with regions that have been shown to contain reiterated sequences.

Keywords: Oryctes rhinoceros, baculovirus, genome insertions and deletions

Received 26 November 1985; accepted 12 February 1986.