| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Unit of Experimental Medicine, Université Catholique de Louvain and International Institute of Cellular and Molecular Pathology, Avenue Hippocrate 75, B-1200 Brussels
and1 Ludwig Institute for Cancer Research, Brussels, Belgium
Mice infected with lactate dehydrogenase-elevating virus (LDV) were found to produce high titres of IgG anti-LDV antibodies that remained elevated for more than 1 year. This response, which was T-dependent, showed a striking preponderance of IgG2a with, from one strain to another, variable proportions of IgG2b and IgG3 but always very little IgG1. The binding of these antibodies to viral protein blots showed a major reaction with VP3, the heterogeneous glycosylated material of the viral envelope. A minor reaction was also noted with VP1, the nucleocapsid protein, but no antibodies were detected against VP2, the non-glycosylated envelope protein of LDV. A similar preponderance of anti-VP3 antibodies was also observed in a large set of anti-LDV hybridomas. Analysis of VP3 with monoclonal antibodies suggested that, despite its heterogeneity, this material has a common polypeptide moiety that apparently carries two major epitopes.
Keywords: LDV, antibodies, isotype, immunoblotting
Received 3 September 1985;
accepted 10 March 1986.
This article has been cited by other articles:
|
|
 |
|
  T. D. Nguyen, G. Bigaignon, D. Markine-Goriaynoff, H. Heremans, T. N. Nguyen, G. Warnier, M. Delmee, M. Warny, S. F. Wolf, C. Uyttenhove, et al. Virulent Toxoplasma gondii strain RH promotes T-cell-independent overproduction of proinflammatory cytokines IL12 and {gamma}-interferon J. Med. Microbiol., October 1, 2003; 52(10): 869 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markine-Goriaynoff, X. Hulhoven, C. L. Cambiaso, P. Monteyne, T. Briet, M.-D. Gonzalez, P. Coulie, and J.-P. Coutelier Natural killer cell activation after infection with lactate dehydrogenase-elevating virus J. Gen. Virol., November 1, 2002; 83(11): 2709 - 2716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markine-Goriaynoff and J.-P. Coutelier Increased Efficacy of the Immunoglobulin G2a Subclass in Antibody-Mediated Protection against Lactate Dehydrogenase-Elevating Virus-Induced Polioencephalomyelitis Revealed with Switch Mutants J. Virol., January 1, 2002; 76(1): 432 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markine-Goriaynoff, J. T.M. van der Logt, C. Truyens, T. D. Nguyen, F. W. A. Heessen, G. Bigaignon, Y. Carlier, and J.-P. Coutelier IFN-{gamma}-independent IgG2a production in mice infected with viruses and parasites Int. Immunol., February 1, 2000; 12(2): 223 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vink, G. Warnier, F. Brombacher, and J.-C. Renauld Interleukin 9-induced In Vivo Expansion of the B-1 Lymphocyte Population J. Exp. Med., May 3, 1999; 189(9): 1413 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Nguyen, G. Bigaignon, J. Van Broeck, M. Vercammen, T. N. Nguyen, M. Delmee, M. Turneer, S. F. Wolf, and J. P. Coutelier Acute and Chronic Phases of Toxoplasma gondii Infection in Mice Modulate the Host Immune Responses Infect. Immun., June 1, 1998; 66(6): 2991 - 2995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Ball, M. E. Hardy, R. L. Atmar, M. E. Conner, and M. K. Estes Oral Immunization with Recombinant Norwalk Virus-Like Particles Induces a Systemic and Mucosal Immune Response in Mice J. Virol., February 1, 1998; 72(2): 1345 - 1353. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
