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J Gen Virol 67 (1986), 1179-1182; DOI 10.1099/0022-1317-67-6-1179
© 1986 Society for General Microbiology
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Differentiation of Serum Antibodies from Pigs Vaccinated or Infected with Aujeszky's Disease Virus by a Competitive Enzyme Immunoassay

J. T. van Oirschot, H. J. Rziha1, P. J. L. M. Moonen, J. M. A. Pol and D. van Zaane

Central Veterinary Institute, Department of Virology, P.O. Box 365, 8200 AJ Lelystad, The Netherlands
and1 Bundesforschungsanstalt für Viruskrankheiten der Tiere, Paul Ehrlich Strasse 28, D-7400 Tübingen, F.R.G.

A competitive enzyme immunoassay was developed to detect antibodies to a glycoprotein (gI) of Aujeszky's disease virus. Infected cell monolayers were used as antigen and a monoclonal antibody directed against an epitope of gI as indicator antibody. It was demonstrated that pigs vaccinated with the Bartha, BUK or NIA-4 strains did not produce antibody to the epitope of gI, whereas all wild-type viruses tested did induce this antibody. The antibody to the gI epitope persisted for at least 15 weeks. The present test, which enables us to distinguish pigs vaccinated with certain attenuated strains from pigs infected with wild-type Aujeszky's disease virus, may be of great value in future combined vaccination-eradication programmes for Aujeszky's disease.

Keywords: antibody differentiation, Aujeszky's disease virus, infection, vaccination

Received 4 December 1985; accepted 20 February 1986.


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