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An RNA-dependent RNA Polymerase Associated with the Filamentous Nucleoproteins of Rice Stripe Virus

Shigemitsu Toriyama

Laboratory of Plant Pathology, Faculty of Agriculture, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

Filamentous particles of rice stripe virus (RSV) were found to be associated with an RNA-dependent RNA polymerase activity. The enzyme catalysed the synthesis in vitro of single- and double-stranded RNAs corresponding in size with RNA found in RSV particles, as well as two other dsRNAs with mol. wt. of 1.0 x 10⁶ and 0.56 x 10⁶. The RSV polymerase activity required Mg²⁺, Mn²⁺ or Fe²⁺ but not Na⁺, K⁺ or NH⁺₄ which were inhibitory at concentrations of more than 50 mM. The optimum pH of the polymerase was around 8·0 at the optimum temperature, 40°C. Detergent treatment of RSV particles did not enhance the polymerase activity. Purified RSV particles contained a minor polypeptide (mol. wt. 230000) as well as coat protein; the former may be the RSV polymerase. The taxonomy of RSV is discussed in relation to enveloped animal viruses with negative-stranded RNA, which also contain an RNA-dependent RNA polymerase in virus particles.

Keywords: RSV, virion polymerase, RNA-dependent RNA polymerase

Present address: National Institute of Agrobiological Resources, Tsukuba Science City-Yatabe, Ibaraki 305, Japan.

Received 22 January 1986; accepted 7 April 1986.