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J Gen Virol 67 (1986), 1361-1371; DOI 10.1099/0022-1317-67-7-1361
© 1986 Society for General Microbiology
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The Myeloproliferative Sarcoma Virus Retains Transforming Functions after Introduction of a Dominant Selectable Marker Gene

Wolfram Ostertag1, Barbara Seliger1,2,, Regine Kollek1, Carol Stocking1, Ulla Bergholz1 and Florence Smadja-Joffe3

1 Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität, Hamburg, Martinistrasse 52, 2000 Hamburg 20, F.R.G.
2 Klinische Arbeitsgruppe ‘Biologische Regulation der Wirt-Tumor-Interaktion’ der Max-Planck-Gesellschaft, Gosslerstrasse 10d, 3400 Göttingen, F.R.G.
and3 Unité 268 INSERM, Oncogénèse Appliquée, Hôpital Paul Brousse, 14 et 16 avenue Paul Vaillant Couturier, 94800 Villejuif, France

The dominant neomycin resistance gene (neoR) was introduced into the genome of the myeloproliferative sarcoma virus (MPSV), a replication-defective retrovirus carrying the mos oncogene. The resulting selectable neoR-MPSV virus did not lose its acute transforming property, unlike the results of attempts by other groups to insert marker genes into oncogenic viruses. NeoR-MPSV DNA was used to generate infectious virus by transfection followed by rescue with Friend or Moloney murine leukaemia virus. Infection of fibroblasts with this virus resulted in morphologically transformed cells which were resistant to the neomycin analogue G418. Segregation of the two functions (transformation and G418 resistance) was not observed in more than 500 independent viral transfers to fibroblasts. Furthermore, neoR-MPSV retained the leukaemogenesis-inducing properties of the wild-type virus. Myeloproliferation and G418-resistance transfer did not segregate after passage in mice.

Keywords: MPSV, retrovirus vectors, transformation, vmos

Received 16 September 1985; accepted 20 March 1986.




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Copyright © 1986 by the Society for General Microbiology.