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J Gen Virol 67 (1986), 1449-1454; DOI 10.1099/0022-1317-67-7-1449
© 1986 Society for General Microbiology
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Integration of Region X of Hepatitis B Virus Genome in Human Primary Hepatocellular Carcinomas Propagated in Nude Mice

Michiko Miyaki1, Chieko Sato1, Tohru Gotanda2, Takane Matsui3, Shunji Mishiro4, Mitsunobu Imai4 and Makoto Mayumi4

1 Biochemistry, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyoku, Tokyo 113, Japan
and2 Hepatitis Division, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyoku, Tokyo 113, Japan
3 Department of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Inadacho, Obihiro 080, Japan
and4 Immunology Division, Jichi Medical School, Minamikawachi, Tochigi-Ken, 329-04, Japan

Tissues of human primary hepatocellular carcinoma (PHC) from six patients infected with hepatitis B virus (HBV) were propagated in nude mice, as well as a strain of hepatitis B surface antigen-positive PHC (PLC/PRF/5). Integration of viral DNA into chromosomal DNA of tumour cells was evaluated by the capacity to hybridize with radiolabelled DNA probes, each representing fundamental partsof the HBV genome, that is S and C genes and regions pre-S and X. All PHC cells possessed region X integrated in their chromosomes. However, integration of the S gene, C gene and region pre-S was found in only six of the seven PHCs. Based on these findings, the integration of region X seems to be most closely associated with carcinogenesis in HBV infection.

Keywords: HBV region X, integration, hepatocellular carcinoma

Received 20 June 1985; accepted 20 March 1986.




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Copyright © 1986 by the Society for General Microbiology.