J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 67 (1986), 1979-1985; DOI 10.1099/0022-1317-67-9-1979
© 1986 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boriskin, Yu. S.
Right arrow Articles by Wild, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boriskin, Yu. S.
Right arrow Articles by Wild, T. F.
Agricola
Right arrow Articles by Boriskin, Yu. S.
Right arrow Articles by Wild, T. F.

Measles Virus Persistent Infection: Modification of the Virus Nucleocapsid Protein

Yu. S. Boriskin1, N. N. Bogomolova1, I. B. Koptyaeva1, P. Giraudon2 and T. F. Wild2

1 Research Institute for Viral Preparations, Moscow 109088, U.S.S.R.
and2 Unité de Virologie Fondamentale et Appliquée, INSERM U.51, Unité CNRS no. 613, 1, place Pr Joseph Renaut, 69371 Lyon Cedex 08, France

The synthesis of intracellular measles virus proteins in persistently infected human cell cultures was studied. The virus-induced proteins were analysed after radioimmuno-precipitation by one- and two-dimensional polyacrylamide gel electrophoresis. The measles virus-induced nucleoprotein (NP) synthesized in persistently infected cells had a reduced binding capacity with measles virus antibodies (human convalescent serum) compared to the NP protein induced by the virus used to initiate the infection. In contrast, monospecific rabbit serum prepared against the original virus NP, or monoclonal anti-NP antibodies, precipitated NP proteins from acutely and persistently infected cells with equal efficiency. When the NP in acutely or persistently infected cells was labelled with either 14C- or 3H-amino acids and subjected to two-dimensional gel analysis, significant charge differences were observed between the virus proteins. When measles virus-infected cells were examined for virus protein synthesis at 40 °C, although no change was found in acutely infected cells, NP was not detected in the persistent infection.

Keywords: measles virus, persistent infection, NP

Received 1 May 1986; accepted 6 June 1986.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1986 by the Society for General Microbiology.