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Transcriptional Map of the Measles Virus Genome

¹ Neurology Service, East Orange VA Medical Center, East Orange, New Jersey 07019, U.S.A.
² Department of Neurosciences, UMDNJ-New Jersey Medical School, Newark, New Jersey 07103, U.S.A.
and³ Department of Microbiology, University of Geneva, C.M.U., 9 avenue de Champel, 1211 Geneva 4, Switzerland

We have constructed a recombinant DNA library of the measles virus genome and identified gene-specific clones containing sequences coding for portions of each of the six viral structural proteins, as well as clones coding for intercistronic sequences. By Northern blotting, we could order the clones according to the pattern of individual gene-specific and readthrough mRNAs. Clones corresponding to the N, P/C and M genes were identified by correlation of the mRNAs with their in vitro translation products; clones corresponding to the H, F and L genes were identified by indirect evidence. The results indicated that the gene order in measles virus is that of a typical paramyxovirus (3'-N,P/C,M,F,H,L-5'), but that the M and F transcripts each contain 1.5 times the coding capacity needed for synthesis of these proteins in vivo.

Keywords: measles virus, gene order, transcription map

Received 18 February 1986; accepted 9 May 1986.

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