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Molecular Cloning of the Double-stranded RNA of Beet Cryptic Viruses

^1* Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands
and² Department of Biochemistry
and³ Department of Plant Pathology, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, U.K.

Three of the four dsRNA components of purified beet cryptic virus (BCV) were copied into cDNA and cloned into pUC9. Clones corresponding to RNAs 1, 3 and 4 did not hybridize to each other or to RNA 2, suggesting that there is no significant sequence homology between the four dsRNA components. RNA extracted from 15 BCV-infected beet plants was analysed by Northern blotting using the cDNA clones as probes. Nine plants were found to contain RNAs 1, 3 and 4 whereas in six plants only RNAs 3 and 4 were detectable. The results are compatible with the occurrence of two different viruses. The sensitivity and specificity of the cDNA hybridization assay was greater than that of immunosorbent electron microscopy in the detection of BCVs.

Keywords: BCV, dsRNA, plant virus detection

Received 20 February 1986; accepted 16 May 1986.