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Respiratory Syncytial Virus Glycoprotein Expression in Human and Simian Cell Lines

¹ Department of Virology, Royal Victoria Infirmary, Queen Victoria Road, Newcastle upon Tyne NE1 4LP
and² Department of Genetics, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, U.K.

Glycoproteins synthesized in both human (HeLa and HEp-2) and simian (Vero and BS-C-1) cell lines following infection with two different strains of respiratory syncytial virus (A2 and Long) were analysed by SDS-PAGE following immunoprecipitation with monoclonal antibodies. Minor virus strain-dependent differences in the large glycoprotein, G, and the fusion protein polypeptides F₁ and F₂ were observed together with minor cell line-dependent differences in the size of the F₂ polypeptide. Major quantities of two glycoproteins, termed Ga (50K) and Gb (45K), were detected in A2 strain-, and to a lesser extent in Long strain-, infected simian cells. These proteins were also present in infected human cells, but in much reduced amounts. Immunoprecipitation with anti-G monoclonal antibodies demonstrated that Ga and Gb shared different epitopes with G.

Keywords: RS virus, glycoproteins, monoclonal antibodies

Received 19 February 1986; accepted 23 May 1986.