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Monoclonal Antibodies to Cowpox Virus: Polypeptide Analysis of Several Major Antigens

¹ Department of Microbiology, Wakayama Medical College, 9-9 Wakayama, 640, Japan
² Department of Clinical Pharmacology, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, Massachusetts 02115, U.S.A.
³ Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka 565, Japan

Monoclonal antibodies directed against major antigens induced by cowpox virus (CPV) were produced. The specificities of these antibodies were established by immunoprecipitation, immunoblotting and several serological analyses, and from the cross-reactivities of these antibodies with cells infected with various other poxviruses, ectromelia virus (EV), vaccinia virus and Shope fibroma virus. The antibodies defined included ones reacting with each of the known major antigens of poxviruses, i.e. the common antigen of all poxviruses (probably NP antigen), the Orthopoxivurs-specific antigen (probably LS antigen), the haemagglutinin, the cell surface antigen, the common A-type inclusions in CPV and EV, and the antigen involved in neutralization.

Received 20 June 1986; accepted 18 September 1986.