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Expression of the Middle Component RNA of Cowpea Mosaic Virus in vivo

Martine Jaegle^1,

¹ Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands
² Institut für Genetik, Universität Köln, D-5000 Köln 41, F.R.G.
and³ Department of Virology, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

Upon infection of cowpea mesophyll protoplasts with cowpea mosaic virus (CPMV), the only M RNA-encoded proteins detected so far have been the two capsid proteins VP37 and VP23. We now report the detection of a M_r 60000 (60K) precursor to both capsid proteins in infected protoplasts cultured in the presence of zinc ions. Furthermore a M RNA-encoded 48K protein was detected in the membrane fraction of infected cells using antisera raised against synthetic peptides. The results obtained indicate that, just like the bottom component RNA, the M RNA of CPMV is translated in vivo into a polyprotein from which proteins are derived by proteolytic cleavage.

Keywords: CPMV, M RNA, capsid protein precursor

Present address: Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ, U.K.

Received 27 April 1987; accepted 25 June 1987.