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Secretion of Particles of Hepatitis B Surface Antigen from Insect Cells Using a Baculovirus Vector

¹ Department of Microbiology and Immunology, University of Ottawa, Faculty of Health Sciences, Ottawa, Ontario, Canada K1H 8M5
² NERC Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.
³ Department of Biochemistry, Seoul National University, College of Medicine, Seoul
and⁴ Korea Green Cross Corporation, Research Laboratories, Seoul, Korea

The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa californica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.

Keywords: HBsAg, baculovirus, AcNPV

Received 9 March 1987; accepted 18 June 1987.