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Characterization of the Genome of Insect Iridescent Virus Type 6 by Physical Mapping

Institut für Medizinische Virologie der Universität Heidelberg, Im Neuenheimer Feld 324, 6900 Heidelberg, F.R.G.

The physical map of the genome (209 kbp) of insect iridescent virus type 6, also known as Chilo iridescent virus (CIV), which is circularly permuted and terminally redundant, was constructed for the restriction enzymes ApaI, Asp718, PvuII and SphI using a gene library of viral DNA containing the complete viral genome. Although the CIV genome is linear, it was found that the restriction maps were circular, because of the unique structure of the genome of this virus.

Keywords: Chilo iridescent virus, genome structure, iridovirus

Received 23 March 1987; accepted 24 June 1987.