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Identification of Viral Structural Proteins in the Nucleoplasm of Potato Yellow Dwarf Virus-infected Cells

¹ Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan 11529
and² Agriculture Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan 40227, Republic of China

Antiserum raised against purified potato yellow dwarf virus (PYDV) reacted specifically with all five viral structural proteins in immunoblots and also with ultrathin sections of infected tobacco cells. In these sections viral proteins were detected with gold-labelled anti-IgG antibodies and gold particles were predominantly located over the nucleoplasm and over PYDV virus particles either at the periphery of nuclei or in the cytoplasm. Control sections of uninfected or healthy cells were not labelled. The results suggest that the nucleoplasm is the only site of accumulation of PYDV structural proteins.

Keywords: PYDV, nucleus, immunogold labelling

Received 2 March 1987; accepted 22 June 1987.