HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Itoh, M. Articles by Homma, M. Search for Related Content PubMed PubMed Citation Articles by Itoh, M. Articles by Homma, M. Agricola Articles by Itoh, M. Articles by Homma, M.

Single Amino Acid Substitution of Sendai Virus at the Cleavage Site of the Fusion Protein Confers Trypsin Resistance

Department of Microbiology, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Kobe 650 Japan
and¹ Department of Viral Infection, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan

Amino acid sequences of fusion (F) proteins of two trypsin-resistant mutants of Sendai virus, TR-2 and TR-5, were deduced from nucleotide analysis of cDNA encoding the F gene and were compared with that of the trypsin-sensitive wild-type Sendai virus. In both mutants, amino acid substitutions were found at residues 116 (Arg Ile), the cleavage site of the F protein, and 109 (Asn Asp). Two trypsinsensitive revertants, TSrev-52 and TSrev-58, derived from TR-5 were both activated by trypsin similarly to the wild-type virus and had a single amino acid reversion from Ile to Arg at residue 116, leaving Asp as before at residue 109. These results indicate that the trypsin sensitivity of Sendai virus can be changed by a single amino acid substitution at the cleavage site of the F protein and a mutation from Arg to Ile is responsible for the acquisition of resistance to trypsin.

Keywords: Sendai virus, trypsin resistant mutant, fusion protein

Received 25 March 1987; accepted 22 July 1987.

This article has been cited by other articles:

				M. Itoh, H. Hotta, and M. Homma Increased Induction of Apoptosis by a Sendai Virus Mutant Is Associated with Attenuation of Mouse Pathogenicity J. Virol., April 1, 1998; 72(4): 2927 - 2934. [Abstract] [Full Text] [PDF]