HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Roggendorf, M. Articles by Rasshofer, R. Search for Related Content PubMed PubMed Citation Articles by Roggendorf, M. Articles by Rasshofer, R. Agricola Articles by Roggendorf, M. Articles by Rasshofer, R.

Characterization of Proteins Associated with Hepatitis Delta Virus

Max von Pettenkofer Institut for Medical Microbiology and Hygiene, University of Munich, Pettenkoferstrasse 9a, D-8000 Munich 2, F.R.G.

The number and size of proteins associated with hepatitis delta virus (HDV) from serum and liver (human, chimpanzee and woodchuck) in the acute and chronic stages of HDV infection were analysed by immunoblotting. HDV particles in serum were separated from serum proteins by gel filtration and peak fractions of HDV antigens were subjected to PAGE. Immunoblotting with human anti-HDV-positive sera and ¹²⁵I-labelled Protein A revealed two bands of 27K and 29K. It was not possible to identify any core-like structure from liver homogenates by CsCl gradient centrifugation. HDV proteins from such gradients were degraded to a size of 14K as determined by immunoblotting. HDV RNA was found in fractions at a density of 1·5 g/ml. However, direct homogenization of liver tissue in gel electrophoresis sample buffer, followed by PAGE and immunoblotting resulted in identification of HDV-associated proteins of 27K and 29K, indicating that HDV proteins in liver tissue are the same size as those in serum, but that they degrade rapidly. There was no difference in size of HDV proteins in liver samples from humans, chimpanzees or woodchucks.

Keywords: HDV, serum, liver

Received 27 January 1987; accepted 14 July 1987.

This article has been cited by other articles:

				L. E. Modahl, T. B. Macnaughton, N. Zhu, D. L. Johnson, and M. M. C. Lai RNA-Dependent Replication and Transcription of Hepatitis Delta Virus RNA Involve Distinct Cellular RNA Polymerases Mol. Cell. Biol., August 15, 2000; 20(16): 6030 - 6039. [Abstract] [Full Text]