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1 Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
and2 Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, U.S.A.
We have characterized a set of 15 monoclonal antibodies to p19gag, one of the internal proteins of avian sarcoma and leukaemia viruses. All the antibodies work in immune precipitations as well as in immunoblotting, though with different efficiencies. We have developed a simple epitope mapping technique, which uses partial chemical cleavages at methionine or tryptophan residues followed by immunoblotting from SDS-polyacrylamide gels, to localize the epitopes of nine of these antibodies. The epitopes fall into at least four classes. The mapping procedure should also be useful for other antigens of known primary structure.
Keywords: ASLV, p19 gag, epitope mapping
Present address: Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, U.S.A.
Received 12 June 1987;
accepted 10 August 1987.
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