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Wheat Streak Mosaic Virus Cylindrical Inclusion Body Protein

Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, Nebraska Agricultural Experiment Station, University of Nebraska, Lincoln, Nebraska 68583, U.S.A.
and¹ Agricultural Biotechnology Laboratories, National Chung Hsing University, Taichung, Taiwan

A protein of apparent molecular weight 66000 was purified from wheat plants infected with wheat streak mosaic virus. Antiserum to this protein, labelled with gold, specifically stained cylindrical inclusions in ultrathin sections of virus-infected cells. Antiserum to the M_r 66000 protein did not react with capsid protein in Western blots, nor did antiserum to capsid protein react with the M_r 66000 protein. Both antisera reacted with homologous antigens. The concentration of the M_r 66000 protein in extracts from infected leaves was about 100 µg per g of leaves, which is higher than the usual concentration of virions.

Keywords: WSMV, cylindrical, inclusion body protein

Received 29 July 1986; accepted 22 October 1986.