HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Meloen, R. H. Articles by Schaaper, W. M. M. Search for Related Content PubMed PubMed Citation Articles by Meloen, R. H. Articles by Schaaper, W. M. M. Agricola Articles by Meloen, R. H. Articles by Schaaper, W. M. M.

Antigenicity and Immunogenicity of Synthetic Peptides of Foot-and-Mouth Disease Virus

Central Veterinary Institute, P.O. Box 65, 8200 AB Lelystad, The Netherlands

Peptides reactive with two neutralizing monoclonal antibodies raised against intact foot-and-mouth disease virus A10 were identified with the aid of all overlapping (hexa)peptides of the outer structural viral protein VP1 and located on the viral surface. Using this procedure, it was possible to define those amino acids within a peptide which were critical in the binding of antibody to that peptide. One eight amino acid long peptide, containing six such amino acids, was virtually indistinguishable from viral antigen in its ability to bind monoclonal antibody as determined by competition tests. Another peptide, which was able to induce neutralizing activity as well, showed no competition and possessed fewer amino acids contributing to binding. This peptide appeared to be an incomplete epitope. Comparison of our data with those of others suggests that this may apply commonly to the reactive peptides described.

Keywords: FMDV, peptides, antigenic epitope

Received 2 June 1986; accepted 15 October 1986.