HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kusters, J. G. Articles by Van der Zeijst, B. A. M. Search for Related Content PubMed PubMed Citation Articles by Kusters, J. G. Articles by Van der Zeijst, B. A. M. Agricola Articles by Kusters, J. G. Articles by Van der Zeijst, B. A. M.

Molecular Epidemiology of Infectious Bronchitis Virus in The Netherlands

Nancy M. C. Bleumink-Pluym^1,

Bernard A. M. Van der Zeijst^1,

¹ Institute of Virology, State University of Utrecht, P.O. Box 80164, 3508 TD Utrecht,
and² Department of Poultry Diseases, State University of Utrecht, P.O. Box 43, 3940 AA Doorn, The Netherlands

Twelve Dutch isolates and the M41 strain of infectious bronchitis virus (IBV), a coronavirus of chickens, were characterized by cross-neutralization and T1 fingerprinting to elucidate their evolutionary relationship. The T1 fingerprinting showed that the Dutch isolates formed two clusters. The first cluster contained strains H52, H120, D387, V1259, V1385 and V1397; the estimated sequence homology is 99%. Cluster two comprised strains D207, D274, D212, D1466, D3128 and D3896, which have about 95% sequence homology. The M41 virus did not belong to either cluster. The four different serotypes which arose in the late 1970s belonged to cluster two and appeared to be different from the vaccine strains (H52 and H120) used at that time. This indicates that the strains were newly introduced and could have arisen from a common virus. On the other hand, three recently isolated field strains were genetically closely related to the vaccine strains H120 and H52 (cluster one), suggesting that these live vaccine strains themselves could have given rise to these serologically altered field isolates. The data are relevant to the development of new vaccine strategies.

Keywords: IBV, epidemiology, genome fingerprinting, serotyping

Present address: Institute of Bacteriology, P.O. Box 80171, 3508 TD Utrecht, The Netherlands.

Received 30 June 1986; accepted 3 November 1986.

This article has been cited by other articles:

				M. F. Ducatez, A. M. Martin, A. A. Owoade, I. O. Olatoye, B. R. Alkali, I. Maikano, C. J. Snoeck, A. Sausy, P. Cordioli, and C. P. Muller Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa J. Gen. Virol., November 1, 2009; 90(11): 2679 - 2685. [Abstract] [Full Text] [PDF]