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1 Istituto di Microbiologia, Università di Roma ‘La Sapienza’, Facoltà di Medicina e Chirurgia, Piazzale Aldo Moro 5,00100 Roma
and2 Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Roma, Italy
A biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (VSV) into host cells of mammalian (HeLa), avian (CER), piscine (EPC) and arthropod (Aedes albopictus) origin, is described. VSV was capable of infecting all cell lines tested by a endsome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. Complement-dependent immune lysis of infected host cells provided evidence that in none of the four different cell types examined did insertion of VSV antigens occur from the outside to any great extent on the cell surface. When the entry process was studied by electron microscopy, virus particles were seen to be bound to the cell surface at 0 °C. After warming at 37 °C for homeothermic cells or at 26 °C for poikilothermic cells, virus was detected within coated pits and coated vesicles and, later, in lysosomes. VSV entry was seen to take place by endocytosis in all four cell lines, which were derived from phylogenetically unrelated species.
Keywords: VSV, endocytosis, entry pathway
Received 17 July 1986;
accepted 15 October 1986.
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