HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ray, R. Articles by Compans, R. W. Search for Related Content PubMed PubMed Citation Articles by Ray, R. Articles by Compans, R. W. Agricola Articles by Ray, R. Articles by Compans, R. W.

Glycoproteins of Human Parainfluenza Virus Type 3: Affinity Purification, Antigenic Characterization and Reconstitution into Lipid Vesicles

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, U.S.A.

Monoclonal antibodies to the envelope glycoproteins, HN and F, of human parainfluenza virus type 3 were coupled to a Sepharose 4B matrix and used for affinity purification of the viral glycoproteins. The purity of the glycoproteins was demonstrated by SDS-PAGE followed by fluorography or silver staining. The antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. The F glycoprotein, when reconstituted into lipid vesicles, showed distinct spike-like projections similar to those of intact virions.

Keywords: parainfluenza virus type 3, envelope glycoproteins, monoclonal antibodies, affinity purification

Received 29 July 1986; accepted 4 November 1986.