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A Herpes Simplex Virus Type 1 Variant which Fails to Synthesize Immediate Early Polypeptide V_mwIE63

MRC Virology Unit, Church Street, Glasgow G11 5JR, U.K.

We report the isolation of a variant (X2D) of herpes simplex virus type 1 strain 17 which has a deletion of 5 x 10⁶ mol. wt. in the long unique and long inverted repeat regions, such that one copy of the immediate early (IE) gene 1 and two unique open reading frames coding for polypeptides of 20K and 22K are deleted. The mutant X2D synthesizes reduced levels of V_mwIE110, and also apparently fails to synthesize V_mwIE63, at both the protein and RNA levels, despite there being no apparent deletion in the coding or controlling regions of the IE2 gene. X2D also fails to synthesize the thymidine kinase polypeptide but exhibits normal growth characteristics in tissue culture.

Keywords: HSV-1, deletion mutant, IR_L

Received 13 October 1986; accepted 19 January 1987.