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1 Laboratory of Molecular Virology, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, U.K.
2 Laboratory of Cellular Oncology, National Cancer Institute and Department of Dermatology, Tufts-New England Medical Centre, Boston, Massachusetts, U.S.A.
and3 Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland, U.S.A.
We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6 protein between HPV-16 and HPV-18, with only one antibody recognizing a cross-reactive epitope. We have used both the monoclonal and the polyclonal antibodies to look for E6 expression in a number of HPV DNA-containing cell lines. These reagents specifically detected a 16.5K mol. wt. polypeptide in cells derived from a human cervical carcinoma.
Keywords: HPV E6, MAbs, cervical carcinoma
Received 2 December 1986;
accepted 2 February 1987.
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