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Time-resolved Fluoroimmunoassay in the Detection of Plant Viruses

¹ Wallac Biochemical Laboratory, Wallac Oy, P.O. Box 10, SF-20101 Turku
and² Agricultural Research Centre, Department of Plant Pathology, SF-31600 Jokioinen, Finland

Time-resolved fluoroimmunoassay (TR-FIA) was used to detect potato virus Y in potato leaf extracts diluted at least 10⁶-fold, and in extracts of secondarily infected dormant tubers diluted 10- or 10²-fold depending on the virus strain used. TR-FIA was also used to detect potato virus X in extracts of secondarily infected tubers diluted up to 10⁶-fold. The response in the TR-FIA using a two-incubation method was linearly related to the virus concentration in a series of dilutions and the method can therefore be used for quantitative analysis. TR-FIA was found to be five to 100 times more sensitive than conventional double antibody sandwich ELISA.

Keywords: time-resolved fluorescence, ELISA, potato virus detection

Received 4 November 1986; accepted 9 February 1987.

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