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A Physical Map of the Pieris rapae Granulosis Virus Genome

Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853, U.S.A.

A physical map for the genome of Pieris rapae (imported cabbageworm) granulosis virus (PrGV) was prepared using the restriction endonucleases BamHI, EcoRI, HindIII, KpnI, SacII and ScaI. The size of the genome was estimated to be 106.5 kbp. PrGV DNA sequences homologous to Trichoplusia ni GV granulin gene sequences and to some Autographa californica nuclear polyhedrosis virus EcoRI fragment sequences were located by Southern blotting. Linear organization of the two baculoviruses was compared.

Keywords: PrGV, physical mapping, DNA homology

Received 19 August 1986; accepted 9 February 1987.