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J Gen Virol 68 (1987), 1485-1490; DOI 10.1099/0022-1317-68-5-1485
© 1987 Society for General Microbiology
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Methylation of Marek's Disease Virus DNA in Chicken T-lymphoblastoid Cell Lines

Akihiro Kanamori1, Kazuyoshi Ikuta1, Shigeharu Ueda1, Shiro Kato1 and Kanji Hirai2

1 Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565
and2 Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11, Japan

Methylation of Marek's disease virus serotype 1 (MDV1) DNA in both productively and latently infected cells was examined by restriction endonuclease analyses with the isoschizomeric pair HpaII and MspI. The latent MDV1 DNA in T-lymphoblastoid cell lines established from chicken T-lymphomas was considerably methylated, whereas methylation of the virus DNA sequences was not detected in productively infected cells.

Keywords: MDV, MD cell lines, methylation

Received 13 October 1986; accepted 20 January 1987.


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Copyright © 1987 by the Society for General Microbiology.