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J Gen Virol 68 (1987), 1543-1551; DOI 10.1099/0022-1317-68-6-1543
© 1987 Society for General Microbiology
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Characterization of Monoclonal Antibodies Against the Human Immunodeficiency Virus (HIV) gag Products and Their Use in Monitoring HIV Isolate Variation

R. B. Ferns, R. S. Tedder and R. A. Weiss1

Virology Section, Department of Medical Microbiology, Middlesex Hospital Medical School, Riding House Street, London W1P 7PN
and1 Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London SW3 6JB, U.K.

Monoclonal antibodies were raised against the gag proteins of a British isolate of the human immunodeficiency virus (HIV), CBL-1. Seven of the monoclonal antibodies recognized HIV gag-coded proteins of 55000 mol. wt. (p55) and 24000 mol. wt. (p24), three recognized p55/p18 and three p18 alone. Cross-competition assays suggested that at least two independent epitopes exist on the p24 cleaved from the p55 precursor, whereas a cluster of closely related epitopes was recognized by the p55/p18 and p18 antibodies. The panel of monoclonal antibodies was then used to compare by immunofluoresence the expression of gag-encoded antigenic determinants on HIV isolates from different geographical locations. Minor changes in epitope expression were observed in isolates from the U.S.A. and Haiti with the most notable changes being detected in isolates from Zaire, Tanzania and Uganda.

Keywords: HIV gag, MAbs, HIV typing

Received 4 December 1986; accepted 12 February 1987.


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