| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Unité de Virologie Fondamentale et Appliquée, INSERM U.51, Unité Associée au CNRS no. 613, 1 place du Professeur Joseph Renaut, 69371 Lyon Cédex 08, France
2 Neurobiology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts, U.S.A.
and3 Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, U.K.
The sequence of the fusion (F) glycoprotein mRNA of the Hallé strain of measles virus was determined from a cDNA clone representing the entire length of the mRNA. It contained 2384 nucleotides, excluding poly(A), with a 5' consensus sequence typical of paramyxoviruses and a 3' terminus found in measles virus mRNAs. The coding sequence was preceded by an unusually long (580 nucleotide) 5' non-translated region, which contained 44% cytosine. The longest open reading frame coded for a polypeptide of 553 amino acids with a predicted molecular weight of 59-84K. Comparison of the sequence with that of the Edmonston strain of measles virus showed that the gene is highly conserved. No amino acid differences were observed between the two strains. The F polypeptide had three regions of high hydrophobicity: an N-terminal signal peptide, the N-terminus of F1 and a C-terminal membrane-spanning region. The four potential asparagine-linked glycosylation sites (one in the signal peptide) were all in the F2 subunit. Comparison of the measles virus F amino acid sequence with other paramyxoviruses revealed homologies with these viruses. Certain regions such as the N terminus of F1 and ten cysteine residues which probably impose structural restraints were highly conserved.
Keywords: measles virus, fusion glycoprotein, nucleotide sequence
Received 5 January 1987;
accepted 23 February 1987.
This article has been cited by other articles:
|
|
 |
|
  M. Takeda, S. Ohno, F. Seki, Y. Nakatsu, M. Tahara, and Y. Yanagi Long Untranslated Regions of the Measles Virus M and F Genes Control Virus Replication and Cytopathogenicity J. Virol., November 15, 2005; 79(22): 14346 - 14354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. Woelk, O. G. Pybus, L. Jin, D. W. G. Brown, and E. C. Holmes Increased positive selection pressure in persistent (SSPE) versus acute measles virus infections J. Gen. Virol., June 1, 2002; 83(6): 1419 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fayolle, B. Verrier, R. Buckland, and T. F. Wild Characterization of a Natural Mutation in an Antigenic Site on the Fusion Protein of Measles Virus That Is Involved in Neutralization J. Virol., January 1, 1999; 73(1): 787 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Caballero, J. Carabana, J. Ortego, R. Fernandez-Munoz, and M. L. Celma Measles Virus Fusion Protein Is Palmitoylated on Transmembrane-Intracytoplasmic Cysteine Residues Which Participate in Cell Fusion J. Virol., October 1, 1998; 72(10): 8198 - 8204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Z. Cleverley and J. Lenard The transmembrane domain in viral fusion: Essential role for a conserved glycine residue in vesicular stomatitis virus G protein PNAS, March 31, 1998; 95(7): 3425 - 3430. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
