HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brown, L. M. Articles by Wood, K. R. Search for Related Content PubMed Articles by Brown, L. M. Articles by Wood, K. R. Agricola Articles by Brown, L. M. Articles by Wood, K. R.

Translation of Clover Yellow Mosaic Virus RNA in Pea Mesophyll Protoplasts and Rabbit Reticulocyte Lysate

Department of Microbiology, University of Birmingham, Birmingham B15 2TT, U.K.

RNA from clover yellow mosaic virus, a potexvirus, was translated in rabbit reticulocyte lysates, and in vitro translation products were compared with virus-specific proteins produced in vivo in pea mesophyll protoplasts inoculated with viral RNA. One major polypeptide with an M_r of 182000 (182K) was produced in vitro, and two were produced in vivo, with M_r 182K and 22K. The 182K product was thought to be the same in vitro as in vivo; the 22K product was shown by Western blotting to be the virus coat protein.

Keywords: CYMV, translation, protoplasts

Received 11 December 1986; accepted 16 March 1987.