HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stussi-Garaud, C. Articles by Godefroy-Colburn, T. Search for Related Content PubMed Articles by Stussi-Garaud, C. Articles by Godefroy-Colburn, T. Agricola Articles by Stussi-Garaud, C. Articles by Godefroy-Colburn, T.

In situ Location of an Alfalfa Mosaic Virus Non-structural Protein in Plant Cell Walls: Correlation with Virus Transport

¹ Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 rue Descartes, 67084 Strasbourg-Cedex
and² Institut National de la Santé et de la Recherche Médicale, Unité 61, 3 avenue Molière, 67200 Strasbourg, France

The 32000 mol. wt. non-structural protein (P3) of alfalfa mosaic virus (AlMV) has previously been shown to accumulate in the cell wall fraction of tobacco leaves infected with AlMV. We now report the ultrastructural location of this protein. P3 was visualized immunocytochemically in the middle lamella of the walls of those parenchymal or epidermal cells which had just been reached by the infection front and in which viral multiplication had just begun. P3 was not found when AlMV had accumulated to high levels in infected cells. These findings support the concept that P3 is involved in the spread of viral infection from cell to cell, i.e. is the transport factor of AlMV.

Keywords: alfalfa mosaic virus, transport, non-structural protein

Received 24 November 1986; accepted 13 February 1987.