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J Gen Virol 68 (1987), 1971-1982; DOI 10.1099/0022-1317-68-7-1971
© 1987 Society for General Microbiology
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Demonstration of Bovine Viral Diarrhoea Virus in Peripheral Blood Mononuclear Cells of Persistently Infected, Clinically Normal Cattle

H. Bielefeldt Ohmann1,{dagger}, L. Rønsholt2 and B. Bloch1

1 Department of Veterinary Virology and Immunology, The Royal Veterinary and Agricultural University, 13 Bulowsvej, DK-1870 Frederiksberg C
and The2 State Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark

Peripheral blood mononuclear cells (PBL) from cattle known to be persistently viraemic with bovine viral diarrhoea virus (BVDV) following a foetal infection, were examined for the presence of viral antigens and cell-associated infectious virus. Using immunocytochemical techniques, physical separations of PBL subsets and virus isolation techniques (directly and by cocultivation) it was found that infection occurred in B and T lymphocytes, monocytes, and a group of cells designated null cells for lack of more specific classification. The latter three groups also supported viral replication, as infectious virus could be isolated from enriched cell populations. BVDV-like particles in cytoplasmic vesicles of PBL subsets were detected by electron microscopy.

Keywords: BVD virus, blood mononuclear cells, persistent infection

{dagger} Present address: Veterinary Infectious Disease Organization, 124 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 0W0.

Received 18 November 1986; accepted 23 March 1987.


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