HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cook, S. D. Articles by Brown, S. M. Search for Related Content PubMed PubMed Citation Articles by Cook, S. D. Articles by Brown, S. M. Agricola Articles by Cook, S. D. Articles by Brown, S. M.

Recovery of Herpes Simplex Virus from the Corneas of Experimentally Infected Rabbits

S. D. Cook^1,2,

¹ MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR
and² Tennent Institute of Ophthalmology, University of Glasgow, Western Infirmary, Glasgow G11 6NT, U.K.

Rabbits were inoculated in the left cornea with one of three strains of herpes simplex virus (HSV) i.e. HSV-1 strain 17, HSV-1 strain McKrae, HSV-2 strain HG52, or with an HSV-1 McKrae/HSV-2 HG52 recombinant R40/2. Fifty-nine to 67 days after inoculation trigeminal ganglia and corneas were explanted and screened for release of infectious virus. Virus was isolated from all left trigeminal ganglia after organ culture irrespective of viral strain. Virus was isolated from three of 16 corneas of animals inoculated with HSV-1 strain McKrae and from one of four corneas from animals inoculated with the HSV-1/HSV-2 recombinant. The isolation of HSV from explanted corneas, after between 15 and 35 days in organ culture suggests that the cornea may be a site additional to dorsal root ganglia where latent HSV can reside in rabbits.

Keywords: HSV, latency, corneal cells

Present address: Department of Ophthalmology, Bristol Eye Hospital, Lower Maudlin Street, Bristol, U.K.

Received 30 January 1987; accepted 15 April 1987.