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Further Characterization of Phage T1 DNA Clones

Department of Genetics, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K.

A series of phage T1 DNA clones containing Sau3A fragments of 1 kb or more were tested for their ability to rescue the defects of amber mutations representing all known T1 genes. Rescue was detected by either complementation or recombination. These data have extended the correlation of the T1 genetic and physical maps and provided more conclusive proof that the large map distances at the left end of the genetic map reflect both increased physical separation of the known genetic markers and enhanced recombination and that the right and left chromosomal ends are recombinogenic to the same extent.

Keywords: phage T1, DNA clones, complementation

Received 12 November 1986; accepted 30 March 1987.