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J Gen Virol 68 (1987), 2153-2160; DOI 10.1099/0022-1317-68-8-2153
© 1987 Society for General Microbiology
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Antigenic Analysis of Human and Bovine Parainfluenza Virus Type 3 Strains with Monoclonal Antibodies

Robert Rydbeck1, Arthur Löve1,3,, Claes Örvell1,2, and Erling Norrby1

1 Department of Virology, Karolinska Institute, School of Medicine, c/o SBL, S-105 21 Stockholm
2 Department of Virology, National Bacteriological Laboratory, S-105 21 Stockholm
and3 Department of Pathology, Division of Neuropathology, Karolinska Institute, Huddinge Hospital, S-146 86 Stockholm, Sweden

The antigenic characteristics of eight human strains and two bovine strains, one of which is represented by two plaque variants, of parainfluenza virus type 3 were analysed. The strains and variants were compared using 52 monoclonal antibodies against five, two, six and six epitopes of the haemagglutinin-neuraminidase (HN), fusion, nucleocapsid and matrix viral proteins respectively, employing radioimmuno-precipitation and immunofluorescence assays. The human strains, seven of which were isolated over 6 years at different geographical locations and the eighth one representing an older prototype strain, showed very little antigenic variation. Extensive differences were detected in all four proteins examined between the human strains and the two strains of bovine origin. Two bovine variants were less effectively neutralized than the prototype human strain with a series of monoclonal antibodies against the HN protein.

Keywords: PIV-3, human, PIV-3, bovine, monoclonal antibodies

Received 11 December 1986; accepted 21 April 1987.




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Copyright © 1987 by the Society for General Microbiology.