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J Gen Virol 68 (1987), 2231-2237; DOI 10.1099/0022-1317-68-8-2231
© 1987 Society for General Microbiology
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Studies on DNA Topoisomerases I and II in Herpes Simplex Virus Type 2-infected Cells

Ashok R. Bapat, Fu-Sheng Han, Zongchao Liu, Bing-Sen Zhou and Yung-Chi Cheng

Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, U.S.A.

It has been suggested that herpes simplex virus (HSV) type 1 may induce a virus-specific DNA topoisomerase activity which copurifies with virus-induced DNA polymerase. We have examined DNA topoisomerase (TOPO) I and II activities in HSV-2-infected HeLa S3 cells. Both activities were partially purified using DEAE-cellulose, phosphocellulose and double-stranded DNA cellulose column chromatography. It was found that both activities could be separated from HSV-2-specific DNA polymerase. Throughout the purification TOPO I could be immunologically detected with a monoclonal antibody developed against human TOPO I. Regardless of the source, mock- or HSV-2-infected human cells, both types of topoisomerase were equally tolerant of 200 mM-KCl. There appeared to be no apparent heterogeneity of TOPO I in HeLa S3 cells through the course of the HSV-2 infection. We conclude that host cell topoisomerases are quite stable in HSV-2-infected HeLa S3 cells and that there is no evidence that HSV-2 is capable of inducing HSV-2-specific TOPO I and TOPO II activities.

Keywords: HSV-2, topoisomerase, DNA polymerase

Received 20 November 1986; accepted 27 April 1987.




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Copyright © 1987 by the Society for General Microbiology.