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Immunoaffinity Purification of a 72K Early Antigen of Human Cytomegalovirus: Analysis of Humoral and Cell-mediated Immunity to the Purified Polypeptide

MRC Clinical Immunology Research Group, Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, London W12 0HS, U.K.

An early antigen of human cytomegalovirus (HCMV) was purified from infected cells as a 72K polypeptide by immunoaffinity chromatography using a monoclonal antibody. It was located in both nucleus and cytoplasm, and was non-glycosylated and undetectable on the surface of infected cells. Known seropositive subjects had antibody against the purified protein and it elicited proliferative T cell responses in 10 of 16 subjects. Five of 14 T cell lines established in response to the purified protein were predominantly CD8⁺ and of these two showed major histocompatibility complex-restricted cytotoxicity against HCMV-infected cells. This provides further evidence that antigens expressed at early times may be targets for the immune response during persistent HCMV infection.

Keywords: CMV, human, early antigens, T cells

Received 16 February 1987; accepted 12 May 1987.

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