J Gen Virol
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J Gen Virol 68 (1987), 2481-2485; DOI 10.1099/0022-1317-68-9-2481
© 1987 Society for General Microbiology
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Quantitative Immunocytochemical Assay for Infectious Avian Retroviruses

Andrew W. Stoker and Mina J. Bissell

Laboratory of Cell Biology, Division of Biology and Medicine, Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720, U.S.A.

A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p19gag or pp60v-src. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.

Keywords: avian, retrovirus, immunocytochemistry

Received 10 February 1987; accepted 13 May 1987.


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