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Recombination of Autographa californica and Rachiplusia ou Nuclear Polyhedrosis Viruses in Galleria mellonella L.

D. Lereclus^2,

¹ Station de Recherches de Pathologie Comparée INRA-CNRS, F-30380 Saint-Christol-lez-Alès
and² Station de Recherches de Lutte Biologique INRA, La Minière, F-78280 Guyancourt, France

Cotransfection of Rachiplusia ou nuclear polyhedrosis virus (RoMNPV) and BamHI or SmaI fragments from the genome of Autographa californica nuclear polyhedrosis virus (AcMNPV) yielded a high frequency of virus recombinants in Galleria mellonella larvae. When larvae were cotransfected either with the entire genome of AcMNPV and RoMNPV restriction fragments or with a mixture of the two viruses, only AcMNPV was produced. In the virus recombinants, the prevalence of certain regions from the genome of the donor virus (AcMNPV) varied considerably, e.g. from 0% in the 35 to 41 map unit region area to a high level in the 20 to 23 map unit region. The AcMNPV polyhedrin gene was shown to be inherited preferentially.

Keywords: AcMNPV, RoMNPV, recombination

Present address: Institut Pasteur, Laboratoire de Biochimie Microbienne, 28 rue du Dr Roux, 75724 Paris, France.

Received 27 February 1987; accepted 7 September 1987.