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Monoclonal Antibodies Against Herpes Simplex Virus Type 1-infected Nuclei Defining and Localizing the ICP8 Protein, 65K DNA-binding Protein and Polypeptides of the ICP35 Family

¹ Institut für Virologie der Freien Universität Berlin
and² Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, D-1000 Berlin 65, F.R.G.

The production and properties of monoclonal antibodies raised against herpes simplex virus type 1 (HSV-1)-infected cell nuclei are described. Biological and immunochemical assays revealed that these antibodies recognize four different proteins in HSV-1-infected cells. Four antibodies reacted with the major DNA-binding protein (ICP8) and six with the 65K DNA-binding protein. Two antibodies detected the ICP35 family of proteins and one antibody bound to a protein with an apparent mol. wt. of 60K. Immune electron microscopy showed that the major DNA-binding protein had a patchy distribution, whereas the 65K DNA-binding protein was evenly spread in the infected cell nuclei. The 60K protein as well as the polypeptides of the ICP35 family were preferentially found associated with the viral capsid.

Keywords: HSV-1, immunolocalization, nuclear proteins

Received 27 May 1987; accepted 28 September 1987.