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Difference in the Production of Human Interferon- and - in Mouse Cells

Miklos I. Toth^1,

¹ Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, H-6701
and² Institute of Microbiology, Medical University of Szeged, H-6720, Hungary

Human interferon-₁ and interferon- genes with their flanking regions were introduced into mouse LMTK^- cells. Although transfected cells contained the interferon genes with a similar copy number and produced a similar amount of interferon-specific mRNA, cells containing the human interferon- gene secreted about 10 times more human interferon than cells transfected with the human interferon-₁ gene. When the coding region of the interferon- gene was replaced by that of the interferon-₁ gene (hybrid interferon / gene), the human interferon production of transfected cells fell by approx. one order of magnitude. These results show that in the case of exogenous interferon genes a translational or post-translational mechanism might significantly affect the final level of human interferons, resulting in higher titres of interferon- than of interferon-.

Keywords: interferon (HuIFN-, -), transfection, regulation

Present address: Institut für Genetik, Universität zu Köln, Weyertal 121, 5000 Köln 41, F.R.G.

Received 9 November 1987; accepted 8 July 1988.