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Integration into the Cellular Genome of a Friend Murine Leukaemia Virus Containing a Selectable Marker Reveals a Clonal Origin of Erythroleukaemia

Norbert Hess^2,

¹ Institute of Medical Virology, Justus-Liebig-University, Frankfurterstrasse 107, D-6300 Giessen
and² Heinrich-Pette-Institute of Experimental Virology and Immunology, Martinistrasse 52, D-2000 Hamburg 20, F.R.G.

We have constructed a selectable Friend murine leukaemia virus (F-MuLV) with a suppressor tRNA (supF) gene integrated into the proviral long terminal repeat. The viral construct was infectious and pathogenic and retained the marker gene when growing in vitro or in vivo. Only a few integration sites of the provirus were detected by Southern blot analysis of the DNA of erythroleukaemic cells. These results indicate that F-MuLV-induced erythroleukaemia is of clonal origin and suggest that insertional mutagenesis is involved in pathogenesis.

Keywords: F-MuLV, suppressor tRNA gene, insertional mutagenesis

Present address: Biology Department, San Diego State University, San Diego, California 92182, U.S.A.

Received 21 April 1988; accepted 8 June 1988.