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Immunocytochemical Location of Pathogenesis-related b₁ Protein Induced in Tobacco Mosaic Virus-infected or Polyacrylic Acid-treated Tobacco Plants

¹ Station de Génétique et d'Amélioration des Plantes
and² Service de Microscopie Electronique, INRA, BV 1540, 21034 Dijon Cédex, France
and³ Department of Plant Pathology, Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, U.K.

Pathogenesis-related b₁ (PR-b₁) protein and other serologically related PR-b proteins, known to be associated with both tobacco mosaic virus infection and chemical treatments, such as with polyacrylic acid (PAA), were detected by immunofluorescence microscopy around epidermal, palisade and lacunar leaf cells in hypersensitive tobacco plants (PAA⁺ line). After gentle fixation and embedding in Lowicryl K4M, PR-b proteins revealed by immunogold labelling were located in the cytoplasm and apoplast. After fixation and embedding in Epon, the PR-b proteins were found to accumulate mainly in the intercellular spaces. These observations support results obtained from biochemical tests.

Keywords: TMV, polyacrylic acid, protein b₁

Received 5 February 1988; accepted 13 July 1988.