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A Virus with a Bisegmented Double-stranded RNA Genome in Rat (Oryzomys nigripes) Intestines

¹ Department of Virology, Instituto Oswaldo Cruz, CP 926, CEP 2001, Rio de Janeiro, Brazil,
² Regional Virus Laboratory, East Birmingham Hospital, Birmingham B9 5ST, U.K.
and³ Department of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

Examination of the intestinal contents of free-living Oryzomys nigripes rats by PAGE revealed two sharply defined bands that could be stained by ethidium bromide or by silver nitrate with comparable intensities. The molecules forming these bands were susceptible to digestion by pancreatic RNase A but not by RNase T1 or by DNase I. Their lengths were estimated to be about 2.6 and 1.5 kbp, respectively, by comparison with rotavirus SA11 genome segments. They cosedimented in CsCl gradients at a density of 1.39 to 1.40 g/ml, together with uniform particles approximately 35 nm in diameter with indistinct surface structure. It is suggested that these particles represent an as yet undescribed virus with a bisegmented double-stranded RNA genome, for which the name ‘picobirnavirus’ is proposed.

Keywords: dsRNA genome, classification, rat virus

Received 4 May 1988; accepted 8 August 1988.

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