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Relationship between the Production of Murine Cytomegalovirus and Interferon in Macrophages

Takahiro Yamaguchi

Yasuhiro Shinagawa

Division of Infectious Diseases, Department of Internal Medicine, University of Texas Medical Branch and Shriners Burns Institute, Galveston, Texas 77550, U.S.A.

Macrophages (M) harvested from the peritoneal cavities of mice after thioglycollate stimulation could be infected with murine cytomegalovirus (MCMV), although the efficiency of infection was low. Sequential measurements of interferon (IFN) production by virus-infected M were performed in an attempt to explain the characteristics of MCMV infection in the cell cultures. Infected M produced moderate amounts of IFN, which was completely neutralized by anti-IFN-/ serum. The IFN was detectable in cultures as early as 8 h after infection and was produced only by exposing M to infectious virus. Production increased until 48 to 72 h and preceded virus production, which was initially detected 72 h after infection. Treatment of the M cultures with anti-IFN-/ resulted not only in a marked increase in virus production, as well as a shortening of the long eclipse period of MCMV infection, but also induced increases in the number of M releasing MCMV (VR-M). Thus, the IFN produced in MCMV-infected M (MCMV-M IFN) appeared to suppress the production and spread of MCMV. The increase in the number of VR-M observed was more resistant to anti-IFN-/ treatment than the production of infectious virus. The antiviral effect of MCMV-M IFN on MCMV infection in mouse embryo fibroblasts was similar to that induced by IFN-/. Therefore, MCMV-M IFN appeared to be more active in protecting against the spread of cell-free MCMV than of cell-associated virus. These differences in sensitivity to IFN action suggest that M may have a role in the latency of MCMV and that their production of IFN may facilitate the generation of latent infection.

Keywords: macrophages, MCMV infection, interferon production

Present address: Department of Radiation Research, Tohuku University School of Medicine, Seirocho 2-1, Sendai 980, Japan.

Received 11 April 1988; accepted 22 August 1988.

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