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Production and Characterization of Bovine Monoclonal Antibodies to Respiratory Syncytial Virus

H. E. Kennedy

E. J. Stott

Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG16 0NN
and¹ Institute of Animal Physiology and Genetics Research, Babraham Hall, Cambridge CB2 4AT, U.K.

Six interspecific hybridomas (heterohybridomas) secreting bovine monoclonal antibodies (MAbs) against respiratory syncytial (RS) virus were produced. Four of the heterohybridomas were formed using the mouse myeloma cell line NS1 as the fusion partner, one using NS0, and the remaining heterohybridoma was formed using a bovine x murine hybridoma as the fusion partner. Five heterohybridomas secreted bovine IgG1 and one secreted IgG2. All six MAbs recognized human subtype A and B viruses as well as bovine RS virus. They were specific for the fusion glycoprotein and reacted with a 140K dimer and a 70K monomer in a Western blot of native antigen; three also bound to the 46K F₁ component and its 22K cleavage product in a blot of reduced antigen. Two of these MAbs neutralized RS virus infectivity, inhibited virus-induced fusion, lysed RS virus-infected cells in the presence of complement and protected mice against RS virus challenge.

Keywords: RS virus, passive immune therapy, heterohybridoma

Present address: Department of Biochemistry, Medical Biology Centre, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, U.K.

Present address: National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, U.K.

Received 6 April 1988; accepted 5 September 1988.

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